Selective exosome exclusion of miR-375 by glioma cells promotes glioma progression by activating the CTGF-EGFR pathway

Abstract Background Exosomes are membrane-bound extracellular vesicles of 40–150 nm in size, that produced by many cell types, and play an important role the maintenance cellular homeostasis. Exosome secretion allows for selective removal harmful substances from cells. However, it remains unclear whether this process also takes place glioma Methods Herein, tumour-suppressor miR-375 was explored human Immunoblotting qRT-PCR experiments demonstrated a functional link between its target, connective tissue growth factor ( CTGF ), which led to identification underlying molecular pathways. The exosomes secreted cells were extracted ultracentrifugation examined transmission electron microscopy. Exosomal expression then analysed qRT-PCR; while exosome inhibitor, GW4869, used examine biological significance release. Moreover, dynamics release investigated using fluorescently labelled exosomes. Finally, exosomal orthotopic xenograft model nude mice. Results MiR-375 downregulated gliomas. suppressed proliferation, migration, invasion inhibiting - epidermal receptor (EGFR) signalling pathway. MiR-375-containing identified peripheral blood samples patients, their level correlated with disease progression status. impacted -EGFR pathway activity. Once secreted, not taken back up Conclusions allowed sustained activation oncogenic pathway, promoting proliferation These findings enhance our understanding biology may inspire development new therapies.